Pine wilt disease, caused by the pinewood nematode Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle, is originating severe infections in pine trees. The disease is detected when external symptoms appear (e.g. needle chlorosis), but trees could remain asymptomatic for long periods and serve as a long-term host. The primary goal of this study was to assess the effect of inoculation with an avirulent isolate of B. xylophilus (C14-5) on different Pinus spp. seedlings (P. sylvestris, P. nigra, P. pinea and P. pinaster). At the same time, seedlings were also inoculated with a virulent strain, HF, in order to compare the phenotypic and genomic results of the two types of inoculations. The effect of inoculation was determined in terms of expression of various Pinus genes potentially involved in the response to the disease.The results suggest that P. pinea and P. nigra are more resistant to infection by the nematode than P. sylvestris and P. pinaster. The phenotypic and genetic differences were more marked among P. pinea and P. pinaster.
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机译:松材线虫Bursaphelenchus xylophilus(Steiner and Buhrer)Nickle引起的松枯病正在松树中引起严重感染。当出现外部症状(例如针叶绿化)时,可以检测到该病,但是树木可以长期保持无症状,可以作为长期宿主。这项研究的主要目的是评估接种无毒的木糖双歧杆菌(C14-5)对不同松属植物的接种效果。幼苗(西番莲,老黑体育,松木和pinaster)。同时,还用强毒株HF接种了幼苗,以比较两种接种的表型和基因组结果。接种的效果是通过表达可能参与该疾病反应的各种松属基因的表达来确定的。结果表明,与樟子松和樟子松相比,P。pinea和P. nigra对线虫感染的抵抗力更高。 。在P. pinea和P. pinaster之间,表型和遗传差异更为明显。
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